Cell Line Authentication: Significance & Types of Errors

A cell line is used in different fields, such as basic cell biology, gene therapy, genetic mapping, and gene expression. Cell lines are critical tools for development and biomedical research. Kaplan and Hukku, in 1998, found that 6% of cell lines examined are cross-contaminated by cells of the same or other species. The exact cell line requires a comprehensive method using multiple complementary procedures that recognize intraspecific & interspecific cell line contaminants. Cell Line Authentication in India is relatively straightforward: DNA profiling can be accomplished through STRs or short tandem repeats. This method makes reproducible data under the guidelines set by leading researchers, scientific organizations, etc. Then, a multiplex polymerase chain reaction generates the short tandem repeats profile followed by capillary electrophoresis on a genetic analyzer.

Need for a Cell Line Authentication Test in India

Three kinds of errors that can be detected are:

• Cross-Contamination- Environmental contaminants like fungus & dust can easily contaminate the cells under research. Maintaining a contamination-free environment in the lab & storage areas is vital to overcome this issue. Despite the care, cell contamination may be inevitable. This test can help the individual in accurately identifying such errors.
• Misidentification- A cell line of interest needs to be misidentified at the source. A specialist would have continued using the same cell for his analysis if it had not been detected. It is a common error while working with cell lines.
• Genetic Drifting- The most vital error with a cell line is that the same cell line may become genetically drifted over time. Furthermore, cells may lose their identity and become different kinds of cells of genetic alteration.

Importance of Cell Line Authentication Test

Cell line authentication tests are critical procedures in various fields, like scientific research, biotechnology, medicine, & forensic investigations. Here are some factors why cell line authentication in India is vital:

• Maintaining Data Integrity: Assures that experiment cell lines are accurately identified and contamination-free. This helps control data inaccuracies & misconceptions due to using the incorrect cell lines.
• Regulatory Obedience: Many regulatory powers require cell line authentication as a standard practice to maintain the validity & integrity of research data.
• Avoiding Cross-Contamination: Detects contamination of cell lines with other cell kinds, which can happen during cell culture, and controls cross-contamination between various cell lines.
• Quality Control: Acts as a quality control measure in labs, verifying the purity and identity of cell lines used in analysis. Authentic cell lines are essential for producing effective biologics, therapeutics, and vaccines.
• Data Reliability: Accurate cell lines generate dependable & reproducible results. Researchers depend on the consistency of cell lines to draw meaningful results and make scientific advances.

Why Choose DNA Forensics Laboratory for Cell Line Authentication in India?

DNA Forensics Laboratory Pvt. Ltd. is one of the most trusted DNA test companies in India. They offer accurate, dependable, & accredited Cell Line Authentication in India at a competitive cost. It’s the only company in India that provides legal DNA tests for various law courts.

Other vital factors that make them among the best companies:

• Have cutting-edge & modern methodologies for the test.
• Send the testing report in 10–15 working days.
• Highly skilled specialists & a team of technical experts carry out each DNA test accurately.
• Have 400+ local & international collection centres for DNA testing
• Their customers are from various biotechnology companies, esteemed research institutes, pharmaceutical companies, etc.